Study of Genes Responsible for Carbapenem Resistance in Pseudomonas aeruginosa Strains Isolated from Local Hospitals ( Najaf Hospitals )

Hind Fieq Abdalameer  Alhachim

doi:10.51699/cajmns.v7i3.3290

Authors

Hind Fieq Abdalameer Alhachim Dept. Pathological analysis, Collage of Science Al-Qadisiya University

DOI:

https://doi.org/10.51699/cajmns.v7i3.3290

Keywords:

Pseudomonas aeruginosa, Carbapenem resistance, Resistance genes, Hospital isolates, Molecular detection

Abstract

It has been associated with a limiting therapeutic options and an increased morbidity in patients hospitalized in critical care.Unit Congregação da Biomedicina MNC, UFMG, Belo Horizonte MG. The objective of this study was to examine the carbapenem resistance genes of P. aeruginosa strains isolated from local hospitals in Najaf , Iraq. Clinical isolates were collected from various wards in the hospital, identified by standard routine microbiological methods of identification with confirmatory molecular techniques. Carbapenem antibiotics resistance patterns were evaluated by antimicrobial susceptibility testing. Polymerase chain reaction targeting major carbapenemase-encoding genes including metallo-beta-lactamase genes and oxacillinase-related genes were used for molecular detection of resistance genes. The results showed the high abundance of carbapenem resistant P. aeruginosa in clinically relevant isolates, and multiple isolates contained one or more resistance determinants. Genes that confer enzymatic inactivation of carbapenems were among the most frequently detected, showing that gene-mediated resistance is a major contributor to local transmission of resistant strains. The finding of several resistance genes in an isolate indicates that multiple mechanisms are involved in reduced susceptibility. These findings underscore the urgent need for continued surveillance, rigorous infection control, and judicious use of antibiotics particularly in hospital settings and identify a previously undocumented reservoir of carbapenem resistance. Moreover, molecular monitoring of resistance genes is critical for informing treatment decisions and preventing further spread of MDR P. aeruginosa in the clinical setting.

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