Estimation of C-Reactive Protein, Calcium, and Glucose Levels in the Blood of Patients with Rheumatoid Arthritis in Salah Al-Din Governorate

Shaimaa Ahmed Saleh

doi:10.51699/cajmns.v7i2.3214

Authors

Shaimaa Ahmed Saleh College of Education for Pure Sciences, Department of Chemistry, Tikrit University, Iraq

DOI:

https://doi.org/10.51699/cajmns.v7i2.3214

Keywords:

C-Reactive Protein, Calcium, Blood Glucose, Rheumatoid Arthritis

Abstract

This study aimed to evaluate serum C-reactive protein (CRP), calcium, and glucose concentrations in patients with rheumatoid arthritis (RA) in Salah al-Din Governorate and to compare these concentrations with those of a healthy control group. The study included 90 samples: 60 persons afflicted with rheumatoid arthritis (RA) and 30 healthy individuals as the control group. We used immunohistochemical, colorimetric, and enzymatic methods to measure the parameters. Then we used statistical analysis to look at the differences and relationships between the variables. The findings indicated a notable increase in CRP levels among patients, particularly in males and the elderly. This proved that CRP is a protein that is present in the acute phase and is a sign of inflammation. As people got older, their calcium levels dropped a lot, but there wasn't much difference between men and women. This is because more calcium is lost and its connection.

References

L. Klareskog, J. Rönnelid, S. Sævarsdóttir, L. Padyukov, and L. Alfredsson, “The importance of differences; On environment and its interactions with genes and immunity in the causation of rheumatoid arthritis,” Journal of Internal Medicine, vol. 287, no. 5, pp. 514–533, May 2020.

D. Aletaha and J. S. Smolen, “Diagnosis and management of rheumatoid arthritis: A review,” JAMA, vol. 320, no. 13, pp. 1360–1372, Oct. 2018.

J. Bullock et al., “Rheumatoid arthritis: A brief overview of the treatment,” Medical Principles and Practice, vol. 27, no. 6, pp. 501–507, Mar. 2019.

B. S. V., “A review article on rheumatoid arthritis (RA),” Asian Journal of Pharmaceutical Research and Development, vol. 14, no. 1, pp. 65–71, Feb. 2026.

T. Pincus, J. R. O'Dell, and J. M. Kremer, “Combination therapy with multiple disease-modifying antirheumatic drugs in rheumatoid arthritis: A preventive strategy,” Annals of Internal Medicine, vol. 131, no. 10, pp. 768–774, Nov. 1999.

A. J. Silman et al., “Twin concordance rates for rheumatoid arthritis: Results from a nationwide study,” Rheumatology, vol. 32, no. 10, pp. 903–907, Oct. 1993.

P. K. Gregersen, J. Silver, and R. J. Winchester, “The shared epitope hypothesis: An approach to understanding the molecular genetics of susceptibility to rheumatoid arthritis,” Arthritis & Rheumatism, vol. 30, no. 11, pp. 1205–1213, Nov. 1987.

C. M. Weyand, K. C. Hicok, D. L. Conn, and J. J. Goronzy, “The influence of HLA-DRB1 genes on disease severity in rheumatoid arthritis,” Annals of Internal Medicine, vol. 117, no. 10, pp. 801–806, Nov. 1992.

M. du Teil Espina et al., “Talk to your gut: The oral-gut microbiome axis and its immunomodulatory role in the etiology of rheumatoid arthritis,” FEMS Microbiology Reviews, vol. 43, no. 1, pp. 1–8, Jan. 2019.

H. Wu et al., “Pathogenic role of tissue-resident memory T cells in autoimmune diseases,” Autoimmunity Reviews, vol. 17, no. 9, pp. 906–911, Sep. 2018.

M. B. Pepys and G. M. Hirschfield, “C-reactive protein: A critical update,” The Journal of Clinical Investigation, vol. 111, no. 12, pp. 1805–1812, Jun. 2003.

P. J. Kennelly, R. F. Murray, V. W. Rodwell, and K. M. Botham, Harper’s Illustrated Biochemistry. New York: McGraw-Hill Medical, 2009.

D. Thompson, M. B. Pepys, and S. P. Wood, “The physiological structure of human C-reactive protein and its complex with phosphocholine,” Structure, vol. 7, no. 2, pp. 169–177, Feb. 1999.

“Calcium level as a predictor of poor outcome in patient with acute ischemic stroke,” International Journal of Science and Research, vol. 6, no. 4, pp. 638–641, 2017.

W. Marshall, “Calcium (serum, plasma, blood),” Association for Clinical Biochemistry, pp. 1–6, 2010.

P. A. Schenck, D. J. Chew, L. A. Nagode, and T. J. Rosol, “Disorders of calcium: Hypercalcemia and hypocalcemia,” in Fluid, Electrolyte, and Acid-Base Disorders in Small Animal Practice, 4th ed., 2006, pp. 120–194.

R. P. Heaney, “Bone mass, nutrition, and other lifestyle factors,” Nutrition Reviews, vol. 54, pp. 53–60, 1996.

C. Higgins, “Urea and the clinical value of measuring blood urea concentration,” Acute Care Testing, pp. 1–6, Aug. 2016.

C. Nita et al., “Fenofibrate improves endothelial function and plasma myeloperoxidase in patients with type 2 diabetes mellitus: An open-label interventional study,” Diabetology & Metabolic Syndrome, vol. 6, no. 1, p. 30, Mar. 2014.

E. D. Harris, Rheumatoid Arthritis. Philadelphia: W. B. Saunders Co., 1997, pp. 3–212.

N. O’Leary, A. Pembroke, and P. F. Duggan, “Single stable reagent (Arsenazo III) for optically robust measurement of calcium in serum and plasma,” Clinical Chemistry, vol. 38, no. 6, pp. 904–908, Jun. 1992.

P. Trinder, “Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor,” Annals of Clinical Biochemistry, vol. 6, no. 1, pp. 24–27, Jan. 1969.

S. Zahim, S. Jameel, and W. Nazhan, “Study of the correlation between serum C-reactive protein and bone biochemical in type 2 diabetic individuals,” Tikrit Journal of Pure Science, vol. 5, p. 19, 2014.

M. J. E. A. Alhial, “Chronic renal failure (uremia syndromes),” Majalat Afaq Altibiyit, no. 10, 2002.

T. Janković et al., “Changes in bone metabolism in patients with rheumatoid arthritis during tumor necrosis factor inhibitor therapy,” Journal of Clinical Medicine, vol. 12, no. 5, p. 1901, Feb. 2023.

P. Oelzner et al., “Relationship between disease activity and serum levels of vitamin D metabolites and PTH in rheumatoid arthritis,” Calcified Tissue International, vol. 62, no. 3, pp. 193–198, Mar. 1998.

S. Boonen, D. Vanderschueren, P. Haentjens, and P. Lips, “Calcium and vitamin D in the prevention and treatment of osteoporosis—A clinical update,” Journal of Internal Medicine, vol. 259, no. 6, pp. 539–552, Jun. 2006.

Z. Tian et al., “The relationship between rheumatoid arthritis and diabetes mellitus: A systematic review and meta-analysis,” Cardiovascular Endocrinology & Metabolism, vol. 10, no. 2, pp. 125–131, Jun. 2021.

D. E. Moller, “Potential role of TNF-alpha in the pathogenesis of insulin resistance and type 2 diabetes,” Trends in Endocrinology & Metabolism, vol. 11, pp. 212–217, 2000.

M. Telford, “The effects of stress on blood sugar composition of the lobster, Homarus americanus,” Canadian Journal of Zoology, vol. 46, no. 5, pp. 819–826, Sep. 1968.

M. J. Peters et al., “Does rheumatoid arthritis equal diabetes mellitus as an independent risk factor for cardiovascular disease? A prospective study,” Arthritis Care & Research, vol. 61, no. 11, pp. 1571–1579, Nov. 2009.