A Study on the Anti – Oxidative Stress Effect Spirulina Supplementation and the Improvement of Certain Biochemical Parameters in Female Rats with Alloxan – Induced Diabetes

Elaf Rabea Ahmed

doi:10.51699/cajmns.v7i4.3390

Authors

Elaf Rabea Ahmed College of Basic Education / Al-Sharqat / Tikrit University / Iraq

DOI:

https://doi.org/10.51699/cajmns.v7i4.3390

Keywords:

Spirulina supplements, diabetes, oxidative stress, biochemical parameters, alloxan

Abstract

This study aimed to evaluate the effects of Spirulina supplementation on oxidative and antioxidant status, as well as on key biochemical parameters—including functional markers and lipid profiles—in alloxan-induced diabetic rats. The study was conducted at Tikrit University over a period of 30 days, using female Wistar rats aged 12–13 weeks and weighing 135–200 g. Diabetes was induced in the animals using alloxan at a dose of 150 mg/kg of body weight. The animals were divided into four groups, with nine animals per group: Group 1 (healthy control group) and Group 2 (diabetic control group). Group 3: A group with induced diabetes treated with 20 ml of a Spirulina solution prepared from 10 g of Spirulina per 100 ml of water. Group 4: A group with induced diabetes treated with 20 ml of a Spirulina solution prepared from 15 g of Spirulina per 100 ml of water. Blood and tissue analyses revealed that the alloxan dose caused weight loss and elevated oxidative stress —driven by free radical production and the depletion and inhibition of antioxidants. This impacted biochemical parameters; specifically, it destroyed pancreatic beta cells and raised liver and kidney enzyme levels, resulting in tissue damage in these organs Compared to the groups that consumed Spirulina supplements, these groups showed weight improvement and a There was a significant decrease) P<0.01 in blood glucose levels, insulin resistance, and glycated hemoglobin, as well as in liver and kidney enzyme levels, accompanied by a marked improvement in the functions of these organs. The results also revealed a significant increase (p0.05> in antioxidant levels— such as glutathione and glutathione peroxidase—and a reduction in malondialdehyde (a marker of oxidative stress).

References

O. Abu Al-Rub, "Diabetes: Symptoms, causes and treatment," 2023.

A. B. Kaiser, N. Zhang, and W. Van Der Pluijm, "Global prevalence of type 2 diabetes over the next ten years (2018–2028)," Diabetes, vol. 67, no. Suppl. 1, p. 202-LB, 2018.

F. F. Ferri, "Diabetes mellitus," ClinicalKey, 2022. [Online]. Available: https://www.clinicalkey.com. [Accessed: May 7, 2022].

A. B. Evert, A. Elson, and E. Nozi, "Nutrition therapy for adults with diabetes or prediabetes: A consensus report," Diabetes Care, 2019, doi: 10.2337/dci19-0014.

Y. Harika, "The best foods to lower blood sugar levels naturally," 2024.

F. Yasmin, "Foods that lower blood sugar," 2020.

S. Liao, "Is Spirulina good for you?" Altibbi, 2024. [Online]. Available: http://www.altibbi.com.

L. Burgess, "What are the benefits of spirulina?" Altibbi, 2024. [Online]. Available: http://www.altibbi.com.

Z. Jing et al., "Phycocyanin and phycocyanobilin from Spirulina platensis protect against diabetic nephropathy by inhibiting oxidative stress," Am. J. Physiol. Regul. Integr. Comp. Physiol., vol. 304, no. 2, pp. R110–R120, 2013, doi: 10.1152/ajpregu.00648.2011.

H. Thormar, "Patented non-antibiotic agents as animal feed additives," Recent Pat. Food, Nutr. Agric., vol. 4, no. 2, pp. 155–168, 2012.

R. Kapoor and U. Mehta, "Supplementary effect of Spirulina on hematological status of rats during pregnancy and lactation," Plant Foods Hum. Nutr., vol. 52, no. 4, pp. 315–324, 1998, doi: 10.1023/A:1008027408919.

V. B. Bhat and K. M. Madyastha, "C-Phycocyanin: A potent peroxyl radical scavenger in vivo and in vitro," Biochem. Biophys. Res. Commun., vol. 275, no. 1, pp. 20–25, 2000.

N. Paniagua-Castro, G. Escalona-Cardoso, D. Hernández-Navarro, R. Pérez-Pastén, and G. Chamorro-Cevallos, "Spirulina (Arthrospira) protects against cadmium-induced teratogenic damage in mice," J. Med. Food, vol. 14, no. 4, pp. 398–404, 2011.

A. Ibrahim and M. Abdel-Daim, "Modulating effects of Spirulina platensis against tilmicosin-induced cardiotoxicity in mice," Cell J., vol. 17, no. 1, pp. 137–144, 2015.

K. Skjånes, R. Aesoy, L. Herfindal, and H. Skomedal, "Bioactive peptides from microalgae: Focus on anti-cancer and immunomodulating activity," Physiol. Plant., vol. 173, no. 2, pp. 612–623, 2021, doi: 10.1111/ppl.13472.

A. Ahmed, A. E. M. El-Mosallamy, N. Nabil, and N. M. E. Abo El-Nasr, "Dose dependent toxicity of streptozotocin inducing diabetic complications in male Sprague Dawley rats with metabolic biochemical histopathological effects," 2025.

S. R. Talbot, M. Heidari, M. W. W., A. Jörns, and O. Naujok, "Comparative analysis of dose response variability and severity in STZ-induced diabetes: Female vs. male NSG mice," Sci. Rep., vol. 16, p. 8257, 2026.

M. S. Miranda, R. G. Cintra, S. B. Barros, and J. Mancini-Filho, "Antioxidant activity of the microalga Spirulina maxima," Braz. J. Med. Biol. Res., vol. 31, no. 8, pp. 1075–1079, 1998, doi: 10.1590/S0100-879X1998000800007.

H. A. A. Rostami, M. Abdoljalal, M. Mohammed, R. Behdad, and M. Majid, "Effect of Spirulina on lipid profile, glucose and malondialdehyde levels in type 2 diabetic patients," Braz. J. Pharm. Sci., vol. 58, p. e191140, 2022.

S. R. Thaakur and B. Jyothi, "Effect of Spirulina maxima on the haloperidol induced tardive dyskinesia and oxidative stress in rats," J. Neural Transm., vol. 114, no. 9, pp. 1217–1221, 2007, doi: 10.1007/s00702-007-0744-2.

M. Gargouri, H. Harmel, A. Akrouti, X. Druart, C. Magné, and A. El Feki, "Effects of Spirulina platensis on lipid peroxidation, antioxidant defenses, and tissue damage in kidney of alloxan-induced diabetic rats," Appl. Physiol. Nutr. Metab., vol. 43, no. 4, pp. 345–354, 2018, doi: 10.1139/apnm-2017-0461.

M. Parviz, E. Nasiri, and H. Sohrabi, "Early renal histological changes in alloxan-induced diabetic rats," Int. J. Med. Cell., vol. 3, no. 1, 2014.

J. H. Chang et al., "Diabetic kidney disease in FVB/NJ Akita mice: Temporal pattern of kidney injury and urinary nephrin excretion," 2012.

K.-M. Choi and H.-S. Yoo, "Amelioration of hyperglycemia-induced nephropathy by 3,3'-diindolylmethane in diabetic mice," Molecules, vol. 24, no. 24, p. 4474, 2019, doi: 10.3390/molecules24244474.

H. Ismail, A. Sakila, S. N. Nahida, W. Ummal, S. B. Abdus, A. Taskina, and R. Kazi, "Reno-pancreas protective effects of Spirulina platensis in alloxan induced diabetic rats," Pak. J. Pharm. Sci., vol. 33, no. 4, 2020.

I. P. Joventino et al., "The microalga Spirulina platensis presents anti-inflammatory action as well as hypoglycemic and hypolipidemic properties in diabetic rats," J. Complement. Integr. Med., vol. 9, no. 1, 2012, doi: 10.1515/1553-3840.1534.

K. M. Sadek, M. A. Lebda, S. M. Nasr, and M. Shoukry, "Spirulina platensis prevents hyperglycemia in rats by modulating gluconeogenesis and apoptosis via modification of oxidative stress and MAPK-pathways," Biomed. Pharmacother., vol. 92, pp. 1085–1094, 2017, doi: 10.1016/j.biopha.2017.06.023.

A. Fayez et al., "Exploring the NRF2/HO-1 and NF-κB pathways: Spirulina nanoparticles as a novel approach to combat diabetic nephropathy," ACS Omega, 2024, doi: 10.1021/acsomega.4c02285.