Development and Evaluation of A New Povidone-Iodine and Glycerin-Based Hydrogel for the Treatment of Infected Wounds: an in Vitro and Animal Study

Ali Hussein Mustafa AL-Obaidi; Majd Ahmed  Jumaah; Qadir Abdullah  Shanak; Mutasim Hisham  Salman

doi:10.51699/cajmns.v7i2.3139

Authors

Ali Hussein Mustafa AL-Obaidi Tikrit University - College of Medicine - Department of Biochemistry - Tikrit-Iraq
Majd Ahmed Jumaah Tikrit University - College of Pharmacy - Department of Pharmaceutical Chemistry - Tikrit-Iraq
Qadir Abdullah Shanak Tikrit University - College of Education for the Pure Sciences - Department of Chemistry - Tikrit-Iraq
Mutasim Hisham Salman Samarra Pharmaceutical Factory (SDI) - Samarra- Iraq

DOI:

https://doi.org/10.51699/cajmns.v7i2.3139

Keywords:

Povidone-Iodine, Glycerol, Wound Healing, Skin Infections, Topical Formulation

Abstract

A novel hydrogel containing povidone-iodine (a potent antiseptic) and glycerol (a moisturizer and anti-inflammatory) was designed and its effects on healing infected wounds and reducing infection-causing bacteria were investigated. The study also evaluated the hydrogel's physicochemical properties, such as its ability to release iodine and glycerol in a controlled manner. Povidone-iodine, known for its antimicrobial properties, was combined with glycerol, a moisturizer that enhances skin hydration and barrier function, to produce a stable and effective topical treatment. This study aimed to develop and evaluate the efficacy of a novel formulation combining povidone-iodine (PVP-G-I) and glycerol for treating skin infections and promoting wound healing. The formulation was prepared by mixing 10% povidone-iodine with purified glycerol in the Central Laboratories of Tikrit University, resulting in a homogeneous mixture. Clinical trials were conducted on rabbit models with wounds. The treated group showed significant wound healing and a reduction in inflammation within days, while the untreated group developed infections. Physical and chemical analyses, including proton nuclear magnetic resonance (1H-NMR) and ultraviolet spectroscopy, confirmed the stability and structural integrity of the formulation, showing no significant changes in its properties over time or at different temperatures. The results demonstrated that the povidone-iodine and glycerol formulation is highly effective in accelerating wound healing, preventing infection, and maintaining skin health without side effects. The study recommends further laboratory and clinical trials to verify its efficacy and safety for human use, highlighting its potential as an effective and cost-effective treatment for dermatological applications.

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