By Green Synthesis Prepared Metal and Metal Oxide Nanoparticles for Medical Application

Hamed A. Gatea; Maithm A. Obaid; Abdulkareem S. Gumar; Auhood Kadhim Zaid

doi:10.51699/cajmns.v7i2.3127

Authors

Hamed A. Gatea Department of Medical Physics, College of Applied Medical Sciences, Al-Shatrah University, Al-Shatrah city, Iraq
Maithm A. Obaid Department of Medical Physics, College of Applied Medical Sciences, Al-Shatrah University, Al-Shatrah city, Iraq
Abdulkareem S. Gumar Department of Medical Physics, College of Applied Medical Sciences, Al-Shatrah University, Al-Shatrah city, Iraq
Auhood Kadhim Zaid Department of Chemistry, College of Science, University of Thi-Qar, 64001, Thi-Qar, Iraq

DOI:

https://doi.org/10.51699/cajmns.v7i2.3127

Keywords:

Principles of Green Chemistry, Nanotechnology, Review of Neonatal

Abstract

Nanotechnology, particularly the development of metal and metal oxide nanoparticles, has significantly advanced medical science through improved drug delivery, diagnostic imaging, and therapeutic applications. However, conventional physical and chemical synthesis methods are often costly, energy-intensive, and environmentally hazardous. This review examines green synthesis approaches that utilize plant extracts, microorganisms, and other biological materials as reducing and stabilizing agents for nanoparticle production. Green methods offer enhanced biocompatibility, reduced toxicity, lower production costs, and improved environmental safety compared to traditional techniques. The paper highlights recent progress in green-synthesized nanoparticles—especially zinc oxide and other metal oxides—for antibacterial, anticancer, and biomedical applications. It also discusses key challenges, including process standardization, large-scale production, regulatory concerns, and long-term toxicity assessment. Overall, green nanotechnology represents a sustainable and promising pathway for advancing modern medicine while aligning with principles of green chemistry and global sustainability goals.

References

M. T. El‐Saadony et al “The Impact of Metal/Metal Oxide Nanoparticle Formation Through Green Synthesis from Plants and Microbes: A Thorough Analysis,” Nanotechnology Perceptions, vol. 20, Aug. 2024, doi: 10.62441/nano-ntp.v20is10.78.

H. F. Etefa, D. J. Nemera, and F. B. Dejene, “Green Synthesis of Nickel Oxide NPs Incorporating Carbon Dots for Antimicrobial Activities,” ACS Omega, vol. 8, no. 41, p. 38418, Oct. 2023, doi: 10.1021/acsomega.3c05204.

K. K. Ilavenil, V. Senthilkumar, and A. Kasthuri, “Green synthesis of metal nanoparticles from three medicinal plants: a review of environmental and health applications,” Discover Catalysis., vol. 2, no. 1. Academy of Medicine, Singapore, Mar. 04, 2025. doi: 10.1007/s44344-025-00007-6.

P. Sharma, N. Bithel, and A. Kaushal, “Green nanoparticle formation toward wound healing, and its application in drug delivery approaches,” European Journal of Medicinal Chemistry Reports, vol. 6, p. 100088, Sep. 2022, doi: 10.1016/j.ejmcr.2022.100088.

A. Hussain et al., “Biogenesis of ZnO nanoparticles using Pandanus odorifer leaf extract: anticancer and antimicrobial activities,” RSC Advances, vol. 9, no. 27, p. 15357, Jan. 2019, doi: 10.1039/c9ra01659g.

M. T. El‐Saadony et al., “Green Synthesis of Zinc Oxide Nanoparticles: Preparation, Characterization, and Biomedical Applications - A Review,” International Journal of Nanomedicine. Dove Medical Press, p. 12889, Dec. 01, 2024. doi: 10.2147/ijn.s487188.

A. M. E. Shafey, “Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: A review,” Green Processing and Synthesis, vol. 9, no. 1. De Gruyter, p. 304, Jun. 18, 2020. doi: 10.1515/gps-2020-0031.

J. Singh, T. Dutta, K. Kim, M. Rawat, P. Samddar, and P. Kumar, “‘Green’ synthesis of metals and their oxide nanoparticles: applications for environmental remediation,” Journal of Nanobiotechnology, vol. 16, no. 1. BioMed Central, Oct. 30, 2018. doi: 10.1186/s12951-018-0408-4.

D. Kavaz, H. Umar, and M. R. Aliyu, “Green Synthesized Metallic Oxide Nanomaterials for Diverse Applications,” NanoWorld Journal, vol. 8, no. 1, Jan. 2022, doi: 10.17756/nwj.2022-096.

A. I. Osman et al., “Synthesis of green nanoparticles for energy, biomedical, environmental, agricultural, and food applications: A review,” Environmental Chemistry Letters, vol. 22, no. 2. Springer Science+Business Media, p. 841, Jan. 20, 2024. doi: 10.1007/s10311-023-01682-3.

H. R. El‐Seedi et al., “Updated Review of Metal Nanoparticles Fabricated by Green Chemistry Using Natural Extracts: Biosynthesis, Mechanisms, and Applications,” Bioengineering, vol. 11, no. 11. Multidisciplinary Digital Publishing Institute, p. 1095, Oct. 30, 2024. doi: 10.3390/bioengineering11111095.

S. Thakur and R. Gupta, “Medicinal plants derived green synthesis of nanoparticles: classification, methods, applications and environmental impacts,” Nanotechnology for Environmental Engineering, vol. 10, no. 2, Apr. 2025, doi: 10.1007/s41204-025-00421-z.

S. Sagadevan, I. Fatimah, J. A. Lett, M. Z. Rahman, E. Léonard, and W. Oh, “Eco-friendly green approach of nickel oxide nanoparticles for biomedical applications,” Open Chemistry, vol. 21, no. 1, Jan. 2023, doi: 10.1515/chem-2023-0141.

P. Rauwel, “Emerging Trends in Nanoparticle Synthesis Using Plant Extracts for Biomedical Applications,” Global Journal of Nanomedicine, vol. 1, no. 3, Mar. 2017, doi: 10.19080/gjn.2017.01.555562.

S. T. Shah et al., “Nature’s nanofactories: biogenic synthesis of metal nanoparticles for sustainable technologies,” Green Chemistry Letters and Reviews, vol. 18, no. 1, Mar. 2025, doi: 10.1080/17518253.2024.2448171.

R. Ullah et al., “A comprehensive overview of fabrication of biogenic multifunctional metal/metal oxide nanoparticles and applications,” Reviews in Inorganic Chemistry, Aug. 2024, doi: 10.1515/revic-2024-0031.

R. Álvarez‐Chimal and J. Arenas-Alatorre, “Green Synthesis of Nanoparticles: A Biological Approach,” in IntechOpen eBooks, IntechOpen, 2023. doi: 10.5772/intechopen.1002203.

V. Soni et al., “Sustainable and green trends in using plant extracts for the synthesis of biogenic metal nanoparticles toward environmental and pharmaceutical advances: A review,” Environmental Research, vol. 202. Elsevier BV, p. 111622, Jul. 07, 2021. doi: 10.1016/j.envres.2021.111622.

K. Hajra, D. Maity, and S. Saha, “Recent Advancements of Metal Oxide Nanoparticles and their Potential Applications: A Review,” Advanced Materials Letters, vol. 15, no. 1. International Association of Advanced Materials, p. 2401, Dec. 20, 2023. doi: 10.5185/amlett.2024.011740.

V. Girotra, P. Kaushik, and D. Vaya, “Exploring sustainable synthesis paths: a comprehensive review of environmentally friendly methods for fabricating nanomaterials through green chemistry approaches,” TURKISH JOURNAL OF CHEMISTRY, vol. 48, no. 5. Scientific and Technological Research Council of Turkey (TUBITAK), p. 703, Oct. 24, 2024. doi: 10.55730/1300-0527.3691.

N. S. Alsaiari et al., “Plant and Microbial Approaches as Green Methods for the Synthesis of Nanomaterials: Synthesis, Applications, and Future Perspectives,” Molecules, vol. 28, no. 1. Multidisciplinary Digital Publishing Institute, p. 463, Jan. 03, 2023. doi: 10.3390/molecules28010463.

J. Iqbal et al., “Green synthesis of zinc oxide nanoparticles using Elaeagnus angustifolia L. leaf extracts and their multiple in vitro biological applications,” Scientific Reports, vol. 11, no. 1, Oct. 2021, doi: 10.1038/s41598-021-99839-z.

A. E. Golli, S. Contreras, and C. Dridi, “Bio-synthesized ZnO nanoparticles and sunlight-driven photocatalysis for environmentally-friendly and sustainable route of synthetic petroleum refinery wastewater treatment,” Scientific Reports, vol. 13, no. 1, Nov. 2023, doi: 10.1038/s41598-023-47554-2.

J. Nandhini, K. Elumalai, and S. Rajeshkumar, “‘Green synthesis of zinc oxide nanoparticles: Eco-friendly advancements for biomedical marvels,’” Resources Chemicals and Materials, vol. 3, no. 4, p. 294, Jun. 2024, doi: 10.1016/j.recm.2024.05.001.

S. Arafat et al., “Phyto-assisted synthesis of zinc oxide nanoparticles using Bauhinia variegata buds extract and evaluation of their multi-faceted biological potentials,” Scientific Reports, vol. 14, no. 1, Sep. 2024, doi: 10.1038/s41598-024-72250-0.

A. Raza et al., “Polyalthia longifolia-mediated green synthesis of zinc oxide nanoparticles: characterization, photocatalytic and antifungal activities,” RSC Advances, vol. 14, no. 25, p. 17535, Jan. 2024, doi: 10.1039/d4ra01035c.

A. Farooq et al., “Green Chemistry Based Synthesis of Zinc Oxide Nanoparticles Using Plant Derivatives of Calotropis gigantea (Giant Milkweed) and Its Biological Applications against Various Bacterial and Fungal Pathogens,” Microorganisms, vol. 10, no. 11, p. 2195, Nov. 2022, doi: 10.3390/microorganisms10112195.

D. Kulkarni et al., “Biofabrication of nanoparticles: sources, synthesis, and biomedical applications,” Frontiers in Bioengineering and Biotechnology, vol. 11. Frontiers Media, May 02, 2023. doi: 10.3389/fbioe.2023.1159193.

E. Matei et al., “ZnO nanostructured matrix as nexus catalysts for the removal of emerging pollutants,” Environmental Science and Pollution Research, vol. 30, no. 54. Springer Science+Business Media, p. 114779, Nov. 03, 2023. doi: 10.1007/s11356-023-30713-3.

G. Dutta, S. kumar Chinnaiyan, A. Sugumaran, and D. Narayanasamy, “Sustainable bioactivity enhancement of ZnO–Ag nanoparticles in antimicrobial, antibiofilm, lung cancer, and photocatalytic applications,” RSC Advances, vol. 13, no. 38, p. 26663, Jan. 2023, doi: 10.1039/d3ra03736c.

D.-M. Radulescu, V.-A. Surdu, A. Ficai, D. Ficai, A. M. Grumezescu, and E. Andronescu, “Green Synthesis of Metal and Metal Oxide Nanoparticles: A Review of the Principles and Biomedical Applications,” International Journal of Molecular Sciences, vol. 24, no. 20. Multidisciplinary Digital Publishing Institute, p. 15397, Oct. 20, 2023. doi: 10.3390/ijms242015397.

H. Singh et al., “Revisiting the Green Synthesis of Nanoparticles: Uncovering Influences of Plant Extracts as Reducing Agents for Enhanced Synthesis Efficiency and Its Biomedical Applications,” International Journal of Nanomedicine. Dove Medical Press, p. 4727, Aug. 01, 2023. doi: 10.2147/ijn.s419369.

B. Naiel, M. Fawzy, A. E. D. Mahmoud, and M. W. A. Halmy, “Sustainable fabrication of dimorphic plant derived ZnO nanoparticles and exploration of their biomedical and environmental potentialities,” Scientific Reports, vol. 14, no. 1, Jun. 2024, doi: 10.1038/s41598-024-63459-0.

M. S. Samuel et al., “A Review on Green Synthesis of Nanoparticles and Their Diverse Biomedical and Environmental Applications,” Catalysts, vol. 12, no. 5. Multidisciplinary Digital Publishing Institute, p. 459, Apr. 20, 2022. doi: 10.3390/catal12050459.

K. Parveen, V. Banse, and L. Ledwani, “Green synthesis of nanoparticles: Their advantages and disadvantages,” AIP conference proceedings, Jan. 2016, doi: 10.1063/1.4945168.

A. Rónavári et al., “Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications,” Molecules, vol. 26, no. 4. Multidisciplinary Digital Publishing Institute, p. 844, Feb. 05, 2021. doi: 10.3390/molecules26040844.

H. J. Fatih, M. Ashengroph, A. Sharifi, and M. M. Zorab, “Green-synthesized α-Fe2O3-nanoparticles as potent antibacterial, anti-biofilm and anti-virulence agent against pathogenic bacteria,” BMC Microbiology, vol. 24, no. 1, p. 535, Dec. 2024, doi: 10.1186/s12866-024-03699-2.

A. B. T. Mawthoh, D. Seram, and H. J. Watt, “Green Synthesized Plant-based Nanotechnology: Cutting Edge Innovation Fostering Sustainability and Revolutionizing Agriculture,” E3S Web of Conferences, vol. 453, p. 1018, Jan. 2023, doi: 10.1051/e3sconf/202345301018.

B. Rajalakshmi et al., “Bio-inspired Nanomaterial’s for Energy Harvesting and Storage: A Green Approach,” E3S Web of Conferences, vol. 552, p. 1122, Jan. 2024, doi: 10.1051/e3sconf/202455201122.

S. Patil and R. Chandrasekaran, “Biogenic nanoparticles: a comprehensive perspective in synthesis, characterization, application and its challenges,” Journal of Genetic Engineering and Biotechnology, vol. 18, no. 1. Elsevier BV, p. 67, Oct. 26, 2020. doi: 10.1186/s43141-020-00081-3.

F. Arshad et al., “Bioinspired and Green Synthesis of Silver Nanoparticles for Medical Applications: A Green Perspective,” Applied Biochemistry and Biotechnology, vol. 196, no. 6. Springer Science+Business Media, p. 3636, Sep. 05, 2023. doi: 10.1007/s12010-023-04719-z.

P. Das, S. Ghosh, and B. Nayak, “Phyto-fabricated Nanoparticles and Their Anti-biofilm Activity: Progress and Current Status,” Frontiers in Nanotechnology, vol. 3, Oct. 2021, doi: 10.3389/fnano.2021.739286.

M. Murali et al., “Zinc oxide nanoparticles prepared through microbial mediated synthesis for therapeutic applications: a possible alternative for plants,” Frontiers in Microbiology, vol. 14. Frontiers Media, Sep. 05, 2023. doi: 10.3389/fmicb.2023.1227951.

A. Sharma et al., “Biogenic Zinc Oxide Nanoparticles: An Insight into the Advancements in Antimicrobial Resistance,” ECS Journal of Solid State Science and Technology, vol. 13, no. 4, p. 47002, Apr. 2024, doi: 10.1149/2162-8777/ad397f.

A. M. Salih et al., “Biosynthesis of zinc oxide nanoparticles using Phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera,” Scientific Reports, vol. 11, no. 1, Sep. 2021, doi: 10.1038/s41598-021-98607-3.

R. Nain, H. Patel, M. Chahar, S. Kumar, D. Rohilla, and M. Pal, “Biosynthesized metallic nanoparticles for sustainable environmental remediation: mechanisms, applications, and future perspectives,” Discover Chemistry., vol. 2, no. 1, May 2025, doi: 10.1007/s44371-025-00203-1.

S. Hosny, G. A. Gaber, M. S. Ragab, M. A. Ragheb, M. Anter, and L. Z. Mohamed, “A Comprehensive Review of Silver Nanoparticles (AgNPs): Synthesis Strategies, Toxicity Concerns, Biomedical Applications, AI-Driven Advancements, Challenges, and Future Perspectives,” Arabian Journal for Science and Engineering, Sep. 2025, doi: 10.1007/s13369-025-10612-0.

S. A. Atanda, R. O. Shaibu, and F. O. Agunbiade, “Nanoparticles in agriculture: balancing food security and environmental sustainability,” Discover Agriculture, vol. 3, no. 1, Feb. 2025, doi: 10.1007/s44279-025-00159-x.

H. R. El‐Seedi et al., “Metal nanoparticles fabricated by green chemistry using natural extracts: biosynthesis, mechanisms, and applications,” RSC Advances, vol. 9, no. 42. Royal Society of Chemistry, p. 24539, Jan. 01, 2019. doi: 10.1039/c9ra02225b.

F. Khan, M. Shariq, M. Asif, M. A. Siddiqui, P. Malan, and F. Ahmad, “Green Nanotechnology: Plant-Mediated Nanoparticle Synthesis and Application,” Nanomaterials, vol. 12, no. 4. Multidisciplinary Digital Publishing Institute, p. 673, Feb. 17, 2022. doi: 10.3390/nano12040673.

H. Saravanan et al., “Exploring nanocomposites for controlling infectious microorganisms: charting the path forward in antimicrobial strategies,” Frontiers in Pharmacology, vol. 14. Frontiers Media, Sep. 27, 2023. doi: 10.3389/fphar.2023.1282073.

U. P. Panigrahy, K. K. Bharti, S. Gupta, and B. Saha, “Development of Eco-Friendly Nanoparticles for Sustainable Drug Delivery: A Comprehensive Review,” Advances in health sciences research/Advances in Health Sciences Research. Atlantis Press, p. 139, Jan. 01, 2025. doi: 10.2991/978-94-6463-813-4_11.