Postoperative Investigation of Serum Biochemical and Immunological Markers in Nephrectomized Rabbits

Najlaa O.H. Aljubury

doi:10.51699/cajmns.v7i1.3069

Authors

Najlaa O.H. Aljubury Department of Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Qadisiyah, Iraq

DOI:

https://doi.org/10.51699/cajmns.v7i1.3069

Keywords:

Kidney Removal, Oryctolagus Cuniculus, Quantitative ELISA, Iraq

Abstract

Nephrectomy, the surgical removal of a kidney, is a significant medical intervention often necessitated by conditions such as renal cell carcinoma, severe trauma, or end-stage renal disease. This study aims experimentally to investigate physiological changes in enzymatic, hormonal and immune markers following nephrectomy in rabbits. A total of 20 adult rabbits were purchased acclimated for one week, and divided randomly into two groups including CG (5 rabbits did not operated surgically) and NG (15 rabbits subjected surgically to full removal of left kidney from each one under aseptic conditions). Sampling of venous blood was performed at different times [preoperative (0), in addition to postoperative 1st, 2nd, 3rd, and 4th weeks) as well as from rabbits of NG at the end of the study (30 days post nephrectomy). After centrifugation, the obtained sera was utilized to measurement of study markers that involved creatine kinase (CK), D-dimer (D2D), erythropoietin (EPO), intercellular adhesion molecule 2 (ICAM-2), interferon-gamma (IFN-g), macrophage inflammatory protein-1 alpha (MIP-1α), tumor necrosis factor-alpha (TNF-α), and tumor necrosis factor-gamma (TNF-g) quantitatively by specific rabbit ELISAs’ kits. In comparison to values of NG, the findings of 0 week were shown insignificant differences (p>0.05) among all study markers. Concerning other times, the findings of CK, D2D, ICAM-2, IFN-g, and MIP-1α were increased significantly (p<0.05) at the 1st, 2nd, 3rd, and 4th weeks. However, the findings of nephrectomy group were revealed a significant reduction (p<0.05) in values 4th week when compared to the 1st, 2nd and 3rd weeks. For TNF-α, though the findings of 1st, 2nd, and 3rd weeks were elevated significantly (p<0.05), the findings of 4th weeks were reduced significantly when compared to 0 week as well as to other weeks of nephrectomy group (1st, 2nd, and 3rd). While values of TNF-g were differed insignificantly (p>0.05) at the 1st week, values of 2nd, 3rd, and 4th weeks were increased significantly (p<0.05). In contrast, significant reduction in values of EPO was identified among the samples of 1st, 2nd, 3rd, and 4th weeks This study indicated that marked physiological changes in enzymes, hormones and immunes markers was occurred due to nephrectomy suggesting the importance of intensive studies on the mechanism and harmful complications. However, complicated interaction between surgical intervention and different biochemical markers highlights the complexity of dealing with patients under various conditions.

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