Determining The Effect of Stratification Methods on The Germination of Tetradium Daniellii Seeds
Abstract
Tetradium daniellii (syn. Evodia hupehensis Dode), commonly known as the bee-bee tree or Korean evodia, is a fast-growing deciduous tree that produces fragrant flowers in the summer, a time when few other trees are in bloom. Due to the extreme hardness of Tetradium daniellii seeds, special pre-sowing treatments were applied. The internal structure of the seeds was studied by preparing anatomical sections. In order to determine the effects of initial treatments—i.e., stratification methods—on seed germination, experiments were conducted. These experiments were carried out using three different variants:1.Control – without any pre-treatment; 2.Four cycles of freezing (-18°C) and thawing (at 24°C, room temperature); 3.Soaking in warm water at 40°C for 24 hours. During the experiments, the germination rate, germination energy, and viability indicators of the seeds under each variant were determined. In this article, laboratory observations were also conducted by placing the seeds in a thermostat at temperatures of +15°C, +20°C, +25°C, and +30°C to determine the effect of temperature on Tetradium daniellii seed germination. The results are presented accordingly.
References
M. Marković, M. Grbić, and D. Skočajić, “Germination of Tetradium daniellii (Benn.) T. G. Hartley seed exposed to different treatments,” 2022, pp. 104–107.
D. S. Poșta, “Research on the use of seed preparation methods for the generative production of seedlings in the species Evodia danielii (Benn.) Hemsl.,” 2015, pp. 54–56.
A. N. B. M. Karim, Z. Ferdous, A. K. Hasan, and A. K. M. M. Rahman, “Performance of mungbean varieties as influenced by date of sowing,” Bangladesh Journal of Agricultural Science, vol. 35, pp. 5–10, 2008.
M. Mamadjanova, B. Karimov, S. Safarova, N. Naralieva, M. Kholmatova, and Z. Yusupov, “Identification and characterization of genetic markers associated with salinity and abiotic stress tolerance in the Tetradium daniellii acclimatized in Uzbekistan,” E3S Web of Conferences, vol. 537, p. 05017, 2024, doi: 10.1051/e3sconf/202453705017.
M. Kim, C. Shim, J. Lee, dan C. Wangchuk, “Hot Water Treatment as Seed Disinfection Techniques for Organic and Eco-Friendly Environmental Agricultural Crop Cultivation,” Agriculture, vol. 12, no. 8, art. no. 1081, 2022.
S. H. A. Al-Hadedy, S. A. Basheer, M. S. Idrees, dan K. A. Y. Al-Taee, “Sulfuric Acid and Hot Water Treatment Effects on the Seed Germination and Growth Traits of Sesbania punicea L.,” SABRAO J. Breed. Genet., vol. 56, no. 1, pp. 444-452, 2024.
S. Surya¬pal Singh, N. K. Bharat, H. Singh, S. Kumar, S. Jakhar, dan Vijay, “Effect of Hot Water Treatment of Seeds on Seed Quality Parameters and Seedling Growth Parameters in Bell Pepper (Capsicum annuum L.) cv. Solan Bharpur,” Indian J. Agric. Sci., vol. 89, no. 1, pp. 133-137, Jan. 2019.
H. P. Msanga dan J. A. Maghembe, “Effect of Hot Water and Chemical Treatments on the Germination of Albizia schimperana Seed,” Forest Ecol. Manage., vol. 17, pp. 137-146, 1986.
Y. Bölek, “Hydropriming and Hot Water-Induced Heat Shock Increase Seed Germination Rate,” Turkish J. Agriculture and Forestry, vol. 37, no. 2, pp. 167-174, 2013.
S. Khan, et al., “Quantifying Temperature and Osmotic Stress Impact on Seed Germination,” Plants, vol. 11, art. no. 900, 2022.
F. L. Luing dan H. Nori, “Cardinal Temperatures and Thermal Time for Germination of Sarawak Traditional Rice,” Malaysian Applied Biology, vol. 52, pp. 1–11, 2023.
H. Khaeim, Z. Kende, I. Balla, C. Gyuricza, A. Eser, dan Á. Tarnawa, “The Effect of Temperature and Water Stresses on Seed Germination and Seedling Growth of Wheat (Triticum aestivum L.),” Sustainability, vol. 14, art. 3887, 2022.
J. Jafari, “Maximizing Tomato Seed Germination,” International Journal of Horticultural Science & Technology, vol. 31, no. 4, pp. 73–85, 2024.
S. Sampayo-Maldonado, et al., “Thermal Time and Cardinal Temperatures for Germination of …,” Forests, vol. 10, no. 10, art. 841, 2019.
B. Jafari, et al., “Temperature Effects on Seed Germination and Seedling Biochemical Profiles of Eight Cannabis Landraces,” MDPI Agriculture, vol. 15, no. 4, art. 73, 2025.
Copyright (c) 2026 M. M. Mamadjanova

This work is licensed under a Creative Commons Attribution 4.0 International License.



