Experimental Evaluation of the Antihypoxic and Antioxidant Activity of the Phytocomposition “Glizimed” in Models of Hypobaric and Hemic Hypoxia
Abstract
This study was conducted to evaluate the antihypoxic and antioxidant effects of the phytocomposition “Glizimed”, prepared from medicinal plants, in experimental models of hypobaric and hemic hypoxia in mice, and to identify possible pathogenetic mechanisms underlying its protective action. Experimental studies were carried out in the vivarium of the Department of Sanitary and Epidemiological Control, Main Medical Directorate, under the Administration of the President of the Republic of Uzbekistan. The experiments involved male albino mice weighing 18–24 g, divided into control and treatment groups. Glizimed was administered intragastrically at doses of 10, 25, and 50 mg/kg. Piracetam (100 mg/kg) and Phytin (200 mg/kg) served as reference drugs. Hypobaric hypoxia was induced by simulating ascent to an altitude of 11,000 m, while hemic hypoxia was modeled by subcutaneous sodium nitrite administration (200 mg/kg). Survival time was recorded as the criterion of antihypoxic activity. Administration of Glizimed significantly increased the lifespan of mice compared with control: by 140.4% under hypobaric hypoxia and by 141.1% under hemic hypoxia. The phytocomposition exhibited superior efficacy compared to Piracetam and Phytin, especially at 25 mg/kg. Glizimed demonstrates pronounced antihypoxic and antioxidant properties in experimental hypoxia, suggesting potential use as a natural pharmacological protector against oxygen deficiency.
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